Ames has areas like Campustown and downtown where walking is convenient, offering access to restaurants, shops, and the university. However, outside these zones, most daily errands will require a vehicle. The city's infrastructure does not uniformly support pedestrian life, making living in strategic areas critical for those preferring to walk.

The city's CyRide bus system, a collaboration involving Iowa State University, offers comprehensive coverage with its 13 fixed routes and Dial-A-Ride service, particularly serving the campus and central areas effectively. This makes it a viable option for travelers, though limited bus frequency demands careful planning for those depending fully on public transit.

With numerous bike trails, designated bike lanes, and resources such as a downloadable bicycle map, Ames supports cyclists well. The city's infrastructure is accommodating for biking, making it a strong mode of transportation for travelers looking to explore the area sustainably, though some may find the road layouts less intuitive in certain neighborhoods.